Estrategia de Control de Oxígeno Disuelto en un Cultivo de Alta Densidad Celular (Effective Dissolved Oxygen Control Strategy for High-Cell-Density Cultures)

Martín Cárcamo (mrcarcam@puc.cl)2, Pedro Saa (pnsaa@puc.cl)2, Jorge Torres (jitorres@puc.cl)2, Samuel Torres (storres@ing.puc.cl)2, Patricio Mandujano (patricio.mandujano@centrovet.cl)1, José Ricardo Pérez Correa (perez@ing.puc.cl)2, Eduardo Agosin (agosin@ing.puc.cl)2


1Centrovet
2Pontificia Universidad Católica de Chile

This paper appears in: Revista IEEE América Latina

Publication Date: May 2014
Volume: 12,   Issue: 3 
ISSN: 1548-0992


Abstract:
Dissolved oxygen control is critical to ensure microorganisms' growth in high-cell-density cultures. Since biological oxygen consumption strongly depends on biomass concentration, high-cell-density cultures demand an increasing and continuous oxygen supply. This is a challenging control problem and one of the major limitations of this type of cultures. The aim of this paper is to provide a simple dissolved oxygen control strategy capable of achieving high biomass levels by manipulating three process variables: agitation, aeration and oxygenation. This strategy was developed using a split-range control scheme specially designed so that the controller action is linear with respect to the maximum transfer rate (kLa•DO*). The controller parameters were tuned through simulation and later implemented and validated in Escherichia coli fed-batch cultivations. The strategy presented here allowed reaching high biomass concentrations while maintaining oxygen concentration at an appropriate level. The implementation of this strategy in high-cell-density cultures will help improve the productivity of aerobic cultures.

Index Terms:
control strategy, split-range, volumetric mass transfer coefficient, high-cell-density cultures   


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